علوم زیستی دریا
zahra masoomi; mohammad zakeri; seied mohammad mousavi; vahid yavari
Abstract
In this study, the effects of different dietary protein and salinity levels on cellular parameters of hemolymph in white leg shrimp (as a part of immune system) of the white leg shrimp (Litopenaeus vannamei) juvenile were studied. The number of 350 shrimps with the average weight of 5.55±0.18 ...
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In this study, the effects of different dietary protein and salinity levels on cellular parameters of hemolymph in white leg shrimp (as a part of immune system) of the white leg shrimp (Litopenaeus vannamei) juvenile were studied. The number of 350 shrimps with the average weight of 5.55±0.18 g and length of 8.81±0.15 cm were distributed randomly in 27 ten tones (length: 600 cm, width: 170 cm and height: 100 cm) concrete tanks. A 3×3 factorial experimental including three levels of salinity (0-3, 12-15 and 32-35 ppt) and three dietary protein levels (25, 35 and 45%). Experimental shrimps were fed to satiation 4 times a day throughout the experimental 56 days. According to the results cellular parameters like: total hemocytes count (THC) and differential hemocyte count (DHC) were analysed and recorded. The dietary protein levels 35% and salinity of 32-35 ppt had direct positive impact on THC and granular cells (GC). Although the semi granular poisonous cells have been decreased with the increase in both of the experimental variables (P≤ 0/05) and no effected on the agranular cells. So based on the results of this study, the protein diet with the 35% is suggested for L. vannamei. Taking in to consideration the results of the study we can say the best water salinity for culture of this species is 32-35 ppt.